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At least 14 people died in an explosion on a trolley-bus in the Russian city of Volgograd in the second attack in the city in as many days.
Investigators described the rush-hour blast as "an act of terror."
They said the bus explosion was caused by a male suicide bomber.
The Federal Investigative Committee said: "The explosive device was set off by a suicide bomber - a man whose body fragments have been collected and sent for genetic testing."
It came just a day after a female suicide bomber was blamed for killing 17 people and leaving dozens more injured at the city's main railway station.
Police identified that bomber as Dagestan national Oksana Aslanova, who had been married to two Islamists killed by Russian forces.
President Vladimir Putin ordered Russia's counter-terrorism agency to step up security nationwide in the wake of the attacks.